Coinlabo: Poids Moléculaire

PM des nucléotides

Nucleotide	MW (g/mol)
Ribonucleotide Triphosphates (Avg. MW = 499.5)
ATP	507.2
CTP	483.2
GTP	523.2
UTP	484.2
Ribonucleotide Monophosphates (Avg. MW = 339.5)
AMP	347.2
CMP	323.2
GMP	363.2
UMP	324.2
Deoxyribonucleotide triphosphates (Avg. MW = 487.0)
dATP	491.2
dCTP	467.2
dGTP	507.2
dTTP	482.2
Deoxyribonucleotide Monophosphates (Avg. MW = 327.0)
dAMP	331.2
dCMP	307.2
dGMP	347.2
dTMP	322.2

PM des acides nucléiques et conversions

Exact M.W. of ssRNA (e.g., RNA Transcript)
M.W. = (A _nx 328.2) + (U _nx 305.2) + (C _nx 304.2) + (G _nx 344.2) + 159
A _n, U _n, C _n, and G _nare the number of each respective nucleotide within the polynucleotide. Addition of "159" to the M.W. takes into account the M.W. of a 5' triphosphate. M.W. calculated is valid at physiological pH.
Exact M.W. of ssDNA (e.g., Oligonucleotides)
M.W. = (A _nx 331.2) + (T _nx 287.2) + (C _nx 288.2) + (G _nx 328.2) + 79.0
A _n, T _n, C _n, and G _nare the number of each respective nucleotide within the polynucleotide. Addition of "79.0" to the M.W. takes into account the 5' monophosphate left by most restriction enzymes. No phosphate is present at the 5' end of strands made by primer extension.
Approximate M.W.'s of Nucleic Acids
M.W. of ssRNA = (# nucleotides x 320.5) + 159.0
M.W. of ssDNA = (# nucleotides x 303.7) + 79.0
M.W. of dsDNA = (# nucleotides x 607.4) + 157.9

Taille et poids: conversions

ssRNA Size and Mass Conversions

Size (nt)	Daltons or g/mol	1 µg equivalent
Size (nt)	Daltons or g/mol	pmol	molecules
20	6,569	152.23	9.17 x 10 ¹³
100	32,209	31.05	1.87 x 10 ¹³
300	96,309	10.38	6.25 x 10 ¹²
500	160,409	6.23	3.75 x 10 ¹²
1000	320,659	3.12	1.88 x 10 ¹²
1800	577,059	1.73	1.04 x 10 ¹²

ssDNA Size and Mass Conversions

Size (nt)	Daltons or g/mol	1 µg equivalent
Size (nt)	Daltons or g/mol	pmol	molecules
20	6,153	162.52	9.79 x 10 ¹³
100	30,449	32.84	1.98 x 10 ¹³
300	91,189	10.97	6.6 x 10 ¹²
500	151,929	6.58	3.96 x 10 ¹²
1000	303,779	3.29	1.98 x 10 ¹²
1800	546,739	1.83	1.1 x 10 ¹²

dsDNA Size and Mass Conversions

Taille (nt)	Daltons ou g/mol	1 µg equivalent
Taille (nt)	Daltons ou g/mol	pmol	molécules
20	12,306	81.26	4.89 x 10¹²
100	60,898	16.42	9.89 x 10¹²
300	182,378	5.48	3.3 x 10¹²
500	303,558	3.29	1.98 x 10¹²
1000	607,558	1.65	9.91 x 10¹²
1800	1,093,478	0.91	5.51 x 10 ¹²

Conversion des unités d'absorbance en masse

1 A ₂₆₀dsDNA = 50 µg/ml
1 A ₂₆₀ssDNA = 37 µg/ml
1 A ₂₆₀ssRNA = 40 µg/ml
Nucleotide Absorbance Maxima and Molar Extinction Coefficients

Nucleotide	Absorbance Maximum at pH 7.0 (nm)	Molar Extinction Coefficient at pH 7.0
ATP	259	15,400
CTP	271	9,000
GTP	253	13,700
UTP	262	10,000
dATP	259	15,200
dCTP	271	9,300
dGTP	253	13,700
dTTP	267	9,600